Mattia Mason, Kaliroi Peqini, Federico Uggeri, Diego Rondelli, Sara Sattin, Luca Pignataro, Sara Pellegrino, Laura Belvisi, Edoardo Scarpa, Raffaella Bucci and Alberto Dal Corso
RSC Chem. Biol., 2025
Check out the latest publication by #APOSlab! 💡
In this work a late-stage peptide functionalization strategy that enables the installation of salicylaldehyde (SA) tags to generate Lys-engaging, reversible-covalent peptide ligands was developed. The strategy was applied to a known binder of NEMO, a key regulator of NFκB signaling. Fluorescence anisotropy assays revealed that several SA-tagged analogues exhibit significantly enhanced affinity compared to the wild-type sequence, consistent with the formation of stabilizing, reversible imine interactions with Lys residues near the peptide-protein interface.
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